摘 要

本文以垂珠花插穗下端2cm的茎作为研究材料，通过取材、固定、脱水、透明、浸蜡、切片、脱蜡、染色、透明等一系列过程的石蜡切片制作及观察，从解剖学的角度观察垂珠花插穗不定根的发育过程，结果表明：①垂珠花1年生插穗茎内无潜伏根原基，不定根由诱生根原基发育形成，诱生根源于愈伤组织自身形成的初生射线与维管形成层交汇处细胞的分裂分化。②大约经30d，不定根原基发育为幼小不定根，并伸出周皮之外。并且愈伤组织内有些细胞分化为加厚的厚壁细胞，在愈伤组织内产生根原基。

关键词：垂珠花；扦插；石蜡切片

Anatomical Observation on the Root System of the Cutting of Styrax dasyanthus

Abstract

This article using Styrax dasyanthus cuttings lower 2cm stems as experimental material,we observed adventitious root of Styrax dasyanthus development from the angle of anatomy trough the materials, fixation, dehydration, transparent, paraffin, slicing, dewaxing and staining, transparent and a series of paraffin section of the process of production and observation. The results were shown as follows: ①there was no incubate root pr-imordium in cutting stem of annual Styrax dasyanthus . The induced root primordium developed into the adventitious root and the adventitious root developed from the originated form, which were generated from the callus of the division and differentiation of the vascular cambium cell. ②After about 30 days, the adventitious root primordia developed into young adventitious roots and extend out of the periderm. The callus could form adventitious root primordium which differentiating into thickening sclerenchyma cells.

Keyword：Styrax dasyanthus ；cuttage；paraffin section

目 录

摘 要 1

前 言............................................................................................................................................3

1.文献综述 4

1.1安息香科植物概况 4

1.2安息香科植物扦插繁殖最新进展.......... 4

1.2.1国内研究成果 4

1.2.2国外相关研究 5

1.3石蜡切片技术 6

2.研究方法与技术路线....................................................................................................................6

2.1研究方法.............................................................................................................................6

2.2技术路线.............................................................................................................................7

2.3本研究的创新之处.............................................................................................................8

3.实验材料及方法 8

3.1实验材料 8

3.2实验准备 8

3.3脱水.....................................................................................................................................8

3.4透明.....................................................................................................................................8

3.5浸蜡与包埋..........................................................................................................................8

3.6切片、烘片和站片..............................................................................................................9

3.7脱蜡......................................................................................................................................9

3.8染色......................................................................................................................................9

3.9透明......................................................................................................................................9

3.10拍照观察............................................................................................................................9

4.实验数据与结果的分析 9

4.1垂珠花扦插生根时期的外部形态发生 9

4.2垂珠花扦插生根过程的解剖学观察................................................................................10

5.小结与讨论...................................................................................................................................21

致谢 22

参考文献 23

前 言

垂珠花属于安息香科，又名小叶硬田螺，为落叶乔木。安息香科（Styracaceae）在全世界约有180种，主要分布于亚洲东部以及北美东南部经墨西哥至安第斯山，在欧洲仅有一种分布于地中海周围。我国约有50种，9个变种，分布广泛，北至辽宁东南部，南至海南岛，东自台湾，西至西藏，主要种类集中于北纬23°-25°，东经100°-120°，垂直分布由50-2500米。本科植物从属来说，在我国分布是全世界最集中的，我国特有种已知的有33种，性状变化十分的大^[1]。

安息香科植物具有重要的药用、经济和观赏价值。安息香是安息香科植物分泌的树脂，含有较多的香脂酸，是珍贵的药材，并可制成高级香料。野茉莉、越南安息香、玉铃花等是良好的蜜源植物，是营造城市园林中蝴蝶栖息地的优良材料，不仅能吸引蝴蝶和蜜蜂等喜欢花蜜的昆虫，为城市园林景观增加生趣，而且也能提高城市生物多样性^[2]。一些乔木树种树干通直且速生，纤维细长，是木材、造纸工业的优良原料。